Glycogen synthase kinase-3 (GSK-3) is a non-receptor protein serine/threonine kinase and a critical modulator of cell fate, neuronal plasticity, and tumorigenesis.
GSK-3 consists of two isoforms, alpha (51kD) and beta (47kD).
Phosphorylation of the kinase activation loop on GSK-3alpha tyrosine 279 (tyrosine 216 for GSK-3beta) by focal adhesion kinase (FAK) or proline-rich/Ca2+-activated tyrosine kinase (Pyk2) activates GSK-3a,b, inhibiting glycogen synthesis and thereby inducing apoptosis.
GSK-3beta also hyperphosphorylates the microtubule- binding protein Tau, leading to paired helical formation, a marker in the pathogenesis of Alzheimer’s Disease.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Controls:Serum starved mouse 3T3-L1 cells +/- insulin (100nM, 10 minutes), or background extracts +/- human recombinant GSK-3beta.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human GSK3a phosphorylated at Tyr279 and GSK3b phosphorylated at Tyr216. Species Crossreactivity: mouse Species Sequence Homology (100%): rat and zebrafish GSK-3 alpha/beta, frog and mouse GSK-3 beta.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to a region of human GSK-3a,b that contains tyrosine 279/216.
