84-1076-98　［取扱停止］Glycogen Synthase 10Blots　G8170-19

特徴

• Glycogen Synthase (GS), is an 85kD protein and a key enzyme in regulating glycogen synthesis by catalyzing the rate-limiting step of UDP-glucose incorporation into glycogen.
• The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise).
• Muscle glycogen synthase is phosphorylated on at least nine sites by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK) and SAPK2b/p38b which leads to its inactivation.
• GSK-3 phosphorylates glycogen synthase at four serine sites in the COOH terminus including serines 641 and 645.
• Serine 641 is also phosphorylated by DYRK, while serine 645 is phosphorylated efficiently by SAPK2b/p38b.
• SAPK2b/p38b is believed to represent a priming kinase that allows GSK-3 to phosphorylate glycogen synthase at serine 641 and therefore inhibits its activity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
• Positive Control:Mouse 3T3-L1 adipocytes lysates; human HepG2 lysates.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.