GATA-4 is a 46kD member of the GATA family of zinc finger-containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling; it plays a critical role in regulating basal and agonist or stress-induced gene expression in cardiac and smooth muscle cell types.
GATA-4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain.
Serine 105 of GATA-4 is phosphorylated in response to agonist stimulation through MEK1-ERK1/2 and weakly through JNK or p38 MAPKs.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Controls Used:Phenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA-4.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
Specificity：Recognizes mouse GATA-4 when phosphorylated at Ser105. Species sequence homology: human, rat and chicken (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from a region of human GATA-4 that contains serine 105.
