Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta (PDGFR beta), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met) and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion.
Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma-1, Nck and SHP2.
Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2 and for activation of the ERK/MAPK pathway in response to EGF.
Applications:Suitable for use in ELISA and Western blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:NIH3T3 cells +/- PDGF.
Gab1 (Grb2-associated binder protein-1) is a ~115kD multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes Human Gab1 when phosphorylated at Tyr627. Species sequence homology: Mouse. Does not cross react with Gab1 phosphorylated at tyrosines 242, 437, 472 or 476.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from the region of human Gab1 that contains tyrosine 627. The sequence is conserved in mouse.
