Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that is a substrate for Src and a key element in growth factor and integrin signaling.
FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
Tyrosine 861 of FAK is a major Src phosphorylation site that promotes the binding of FAK to the cytoplasmic tail integrins and is also involved in cancer.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilution determined by the researcher.
Positive Control:Chicken embryo fibroblasts expressing FAK protein and plated on fibronectin.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human FAK when phosphorylated at Tyr861, at ~125kD.. Species Crossreactivity: mouse and chicken. Species sequence homology: rat and frog.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ch Hu Mo
Immunogen：Synthetic phosphopeptide derived from the region of human FAK that contains tyrosine 861.
