Focal Adhesion Kinase (FAK) is a 125kD non-receptor protein tyrosine kinase that was discovered as a substrate for Src and is a key element of integrin signaling.
FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
Phosphorylation of tyrosine 407 is activated by TGFb and Epithelial Mesenchyme Transition (EMT).
Unlike other sites on FAK, tyrosine 407 is EGFR independent.
Applications:Suitable for use in Western blot, Immunocytochemistry andImmunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilution determined by the researcher.
Positive Control:Chicken embryo fibroblast (CEF) cells expressing FAK protein and plated on fibronectin.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human FAK when phosphoryated at Tyr407. Species Crossreactivity: mouse, rat, Species sequence homology: chicken, 100%; frog, 92%
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from the region of human FAK that contains tyrosine 407.
