Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor.
The activity of eIF2B is regulated by a wide array of conditions.
Stress inhibits its action, while stimulation of protein synthesis results in its activation.
eIF2Bε, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Recommended Positive Controls:Recombinant eIF2Bε treated with GSK-3b; CHO-T cells transfected with human insulin receptor (IR) +/- insulin.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes rat eIF2Bε when phosphorylated at Ser539. Species sequence homology: human (80%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic phosphopeptide derived from the region of rat eIF2Bε that contains serine 535 (corresponding to serine 539 in the human sequence).
