eIF2a is a 36kD protein which is ubiquitously expressed in many cell types.
The eIF2 protein, which is composed of three subunits (a, b and g), is one of the key molecules in the initiation of translation.
In mammalian cells, stress induces eIF2a to be phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR).
Phosphorylation of eIF2a blocks the GDP-GTP exchange activity of eIF2b, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction.
The phosphorylation of eIF2a is an important regulatory process in protein synthesis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human eIF2a when phosphorylated at Ser52. Species Crossreactivity: Mouse. Species Sequence Homology: Rat, porcine, bovine, Drosophila, and yeast.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from the region of human eIF2a that contains serine 52.
