The proto-oncogene ETS1 (E-twenty-six-specific sequence) is the founding member of a family of transcription factors that share a highly conserved DNA-binding domain, called the ETS domain, which recognizes a nucleotide motif with a GGAA/T core sequence.
The ETS genes are dispersed on two separate chromosomal loci called ets-1, which codes for a 54kD protein and ets-2, which codes for a 56kD protein.
They are responsible for the regulation of critical genes involved in cell proliferation, differentiation, lymphoid development, motility, invasion, angiogenesis and apoptosis.
ETS1 has been shown to be phosphorylated on serine 251, 257, 282 and 285 by calmodulin-dependent kinase II in vitro and following antigenic stimulation of T or B lymphocytes or treatment with calcium ionophores in vivo.
Phosphorylation at these sites, which are adjacent to the DNA binding domain, has been shown to inhibit ETS1 binding to specific DNA sequences but does not affect ETS1 localization to the nucleus.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human ETS1 when phosphorylated at Ser282. Species sequence homology: Chicken, pufferfish, frog, mouse, rat (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Fr Hu Mo Xe
Immunogen：Synthetic phosphopeptide corresponding to human ETS1 that contains serine 282.
