Cofilin is a 20kD actin-binding protein and a key regulator of actin dynamics involved inregulating lamellipodia formation, neurite extension, chemotaxis and tumor cell migration
Phosphorylation of cofilin by LIM kinase (LIMK1) on serine 3 mediates Rho-ROCK and Rac-PAK signaling pathways in regulating actin reorganization by inactivating cofilin and blocking its ability to promote actin-filament disassembly.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:MDCK cells treated with staurosporine; Jurkat cells treated with hydrogen peroxide.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol, 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide.
Specificity：Recognizes human and canine cofilin phosphorylated at Ser3. Species sequence homology: mouse and rat (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from a region of human cofilin that contains serine 3. Species sequence homology: mouse and rat.
