Mammalian cyclin-dependent protein kinase 1 (cdk1)-also known as cdc2 in yeast-is the catalytic portion of a cyclin/cdk complex.
It is known to associate with both cyclins A and B.
Upon activation, cdk1 induces cells to progress from the G2 phase of the cell cycle through mitosis.
This occurs when threonine 14 and tyrosine 15 are dephosphorylated in the presence of the phosphatase Cdc25.
This relieves the inhibition on cdk1 phosphorylated on threonine 161, allowing it to become active.
Myt1 and wee1 act to re-phosphorylate threonine 14 and tyrosine 15, causing inactivation.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cells treated with nocozadole
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human cdk1 when phosphorylated at threonine 14 and tyrosine 15. Does not recognize non-phosphorylated Thr15, Tyr15 cdk1. May recognize other cyclin-dependent kinases phosphorylated on the homologous residues. Does not recognize cdk4 or cdk6. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from the region of human cyclin-dependent kinase that contains threonine 14 and tyrosine 15.
