b-catenin (92kD) is a multifunctional protein involved in cell-cell adhesion, intracellularsignaling and gene transcription.
b-catenin plays a key role in the Wnt pathway which mediates numerous developmental processes.
Deregulation of the Wnt pathway results in the accumulation of b-catenin in the nucleus.
Stabilizing mutations of several serine/threonine residues (more frequently of serine 45) have been found in multiple tumors.
Phosphorylation of b-catenin on serine 45 by casein kinase I (CKI) primes its subsequent phosphorylation of residues 33, 37 and 41 by glycogen synthase-3b (GSK-3b) and results in its degradation.
Applications:Suitable for use in ELISA and Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human b-catenin when phosphorylated at Ser45. Species sequence homology: mouse, rat and frog b-catenin (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human b-catenin contains serine 45. Species sequence homology: mouse, rat andfrog.
