Calcium/calmodulin-dependent protein kinase II a (CaMKIIa) is a 50kD member of the CaMKII family of serine-threonine kinases that transduce Ca2+ signals to several target proteins, including ion channels and transcription activators.
CaMKII is predominantly expressed in two isoforms in the brain: a and b.
CaMKII plays an important role in neuronal plasticity and memory formation, and exerts both calcium-calmodulin-dependent and -independent activities.
Autophosphorylation of CaMKIIa on threonine 286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state, and is required for various cellular functions including hippocampal long-term potentiation (LTP), special learning, and hippocampus-dependent memory.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes Rat CaMKIIa when phosphorylated at Thr286. Species sequence homology: human and mouse (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phosphopeptide derived from a region of human CaMKIIa contains threonine 286. Species sequence homology: mouse and rat.
