Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family.
There are three isoforms of Bim: BimEL, BimL and BimS.
Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3.
Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation.
BimEL, the long isoform of Bim, is a ~28kD protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway.
The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:Hek293T co-expressing rat BimEL and active MEKK1.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes rat BimEL. Species Crossreactivity: human BimEL when phosphorylated at serine 69.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rt
Immunogen：Phosphopeptide corresponding to rat BimEL that contains serine 65 (serine 69 in the human sequence). Species sequence homology: mouse (100%).
