Bcl-xL is a ~28kD member of Bcl-2 family of proteins and an important regulator of apoptosis.
Bcl-xL forms heterodimers with BAX, BAK and Bcl-2 and its overexpression in tumor cells confers resistance against chemotherapeutic drugs.
Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine.
Phosphorylation of serine 62 – thought to be mediated by Jun N-terminal stress kinase (JNK) signaling – negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:SK-BR-3 cells treated with vinblastine.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Bcl-xL when phosphorylated at Ser62. Species sequence homology: mouse and rat Bcl-xL (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human Bcl-xL that contains serine 62. Species sequence homology: mouse and rat.
