Akt, also known as Protein Kinase B (PKB) or RAC-a, is a 60kD serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3b, caspase 9, BAD, and the Forkhead Transcription Factor.
Akt is activated by PI3K, which in turn can interact with proteins like FAK (when FAK is phosphorylated at tyrosine 397), thereby linking activation to the cytoskeleton.
Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2.
Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of Akt.
Applications:Suitable for use in ELISA and Western Blot Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Akt1 when phosphorylated at Thr308. Species Crossreactivity: Mouse. Species sequence homology: Other species, Akt2 (100%), Akt3 (92%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the region of human Akt1 that contains threonine 308.
