AS160 is a 160kD Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4.
AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking.
Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function.
Phosphorylation of threonine 642 is also important in mediating AS160 redistribution from low density microsomes to the cytosol in adipocytes.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:3T3-L1 adipocytes or COS cells overexpressing AS160, treated with insulin.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified from rabbit serum by affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human AS160 when phosphorylated at Thr642. Species crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：AS160 derived peptide, phosphorylated at threonine 642.
