84-1076-64　Aquaporin 1 (AQP1, CHIP28) (Biotin) (Azide, BSA & Glycerol free) 50ul　A3000C

特徴

• Water is a critical component of all living cells.
• Interestingly, tissue membranes show a great degree of water permeability.
• Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
• Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
• Aquaporin, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells.
• Over the last decade, genes for several members of the aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
• Aquaporin 1 (APQ1) is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules.
• AQP1 is also expressed by the choroid plexus and various other tissues.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: Chemiluminescence technique reveals two bands, a lower 28kD band corresponding to unglycosylated form and an upper high molecular 35-60kD glycosylated component (Denker et al.
• 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
• Optimal dilution determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.