Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot (Rat kidney membranes): 1:400-1:800Immunohistochemistry: Rat kidney sections
Optimal dilution determined by the researcher.
Control Antigen:A3000A: Aquaporin 1 (AQP1, CHIP28), Control Antigen
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer
Note: We do not recommended adding glycerol as a cryopreservative.
仕様
Size:50ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol free.
Specificity:Recognizes rat Aquaporin 1 at ~28.8kD.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Rt
Immunogen:Synthetic peptide corresponding to 19aa from the cytosolic carboxyl terminal domain of the AQP1, conjugated to KLH. This peptide contains most of the epitopes recognized by polyclonal antibodies against the whole protein (NP_036910.1). Species Sequence Homology: human, mouse, bovine, sheep: 100%