Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focaladhesions, which are critical to cell adhesion and migration.
This in turn plays a role in a widevariety of processes including embryogenesis, organogenesis, wound repair, inflammation andcancer.
Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve asdocking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk2, Src), serine/threoninekinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk).
Tyrosine31, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:NMuMG cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes mouse paxillin when phosphorylated at Tyr31. Species sequence homology: Human (100%) and chicken (93%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to human paxillin that contains tyrosine 31.
