84-1076-61　Aquaporin 2 (AQP-CD, WCH-CD) (Azide, BSA & Glycerol free) 50ul　A3000-07K1

特徴

• Water is a critical component of all living cells.
• Interestingly, tissue membranes show a great degree of water permeability.
• Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
• Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”.
• Over the last decade, genes for several members of aquaporin family (AQP1-9 and AQPAP) have been cloned, expressed, and their distribution studied in many tissues.
• AQP2 (AQP-CD or WCH-CD; 271aa; human gene locus 12q13) is the vasopressin-regulated water channel of the apical membrane of collecting duct cells.
• AQP families of proteins are predicted to contain six transmembrane domains.
• The N- and C-terminus are predicted to be cytoplasmic.
• Defects in AQP2 are a cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).
• Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL)
• ELISA: 1:10,000-1:50,000; Coat plates with control peptide at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:A3000-07M: Aquaporin 2, Rat Control Peptide
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.