TNF-R II is present on most cell types and is considered to play a role in cell stimulation by TNF-alpha.
TNF-R II molecule is shown to be responsible for stimulation of activated T-lymphocytes by TNF-alpha.
Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunoprecipitation.
The antibody is an agonistic antibody useful for detection of TNF-R II.
The reactivity of the antibodies with cellbound or soluble TNF-Receptor is not inhibited by high concentrations of TNF-alpha.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10Flow Cytometry: 1:10Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity：Recognizes the extracellular part of human TNF-R II and with the soluble receptor. Minimal crossreactivity is present with human TNF-alpha.
