Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts.
It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2.
TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections.
TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.
Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry and Neutralization studies.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 1:10Western Blot: 1:10Neutralization: biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes natural and recombinant human TNF-alpha. Inhibits the biological activity of human natural and recombinant TNF-alpha as determined with L929 and WEHI cells in a cytotoxicity assay. Species Crossreactivity: rhesus and cynomolgous natural TNF-alpha. Does not crossreact with human lymphotoxin.
