Tumor Necrosis Factor alpha (TNF-alpha) is a cytokine which has diverse immunomodulatory, anti-tumor and toxic effects.
TNF-alpha has been detected in diverse inflammatory status and appears to be a critical mediator in the lethality of septic shock.
Furthermore, TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA, Western Blot and Neutralization.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (frozen and paraffin): 1:10Flow Cytometry: 1:10Western Blot: 1:10Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, 0.1% BSA.
Specificity:Recognizes native and recombinant mouse TNF-alpha and recognizes membrane and receptor bound TNF-alpha. Shows neutralizing activity. Species Crossreactivity: rat.