IL-10 is a pluripotent cytokine with important immunosuppressive actions: it can inhibit cytokines involved in the Th1 response such as IL-2, Interferon gamma and also inhibits the production of pro-inflammatory cytokines such as IL-1, IL-6, IL-8 and TNF-alpha.
Applications:Suitable for use in Western Blot, ELISA and for Neutralization of human IL-10 in bio-assays.
Furthermore the antibody can be used to Immunocytochemically stain cells containing IL-10 in frozen sections as well as in cells cultured on cover slips.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 1:10For neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-10 to be inactivated.
Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Please inquire for availability of azide free solutionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity：Recognizes human Interleukin-10 (IL-10) of both natural and recombinant origin. Inhibits the biological activity of natural and recombinant human IL-10. Specificity was further assessed by ELISA. No crossreactivities with other cytokines have been detected.
