IFN-gamma is a pluripotent cytokine with important pro-inflammatory functions.
Applications:Useful for Neutralization of human IFN-gamma in bio-assays.
Furthermore the antibodies can be used for Western Blot, ELISA and to Immunohistochemically stain cells containing IFN-gamma in frozen sections as well as in cells cultured on cover slips.
Recommended Dilution:Immunohistochemistry: 1:10Western Blot: 1:10For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated.
Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Please inquire for availability of azide free solutions
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
Specificity：Recognizes human gamma IFN (IFN-gamma) of both natural and recombinant origin. Inhibits the biological activity of natural and recombinant human IFN-gamma. Specificity was further assessed by ELISA and Western Blot. No crossreactivities with other cytokines have been detected.
