IFN-gamma is a pluripotent cytokine with important pro-inflammatory functions.
Applications:Can be used for Neutralization in bio-assays.
Furthermore the antibody is useful for Immunohistochemical staining of IFN-gamma containing cells in frozen sections as well as in cells cultured on cover slips and for Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:10Western Blot: 1:10For neutralization of biological activity dilutions have to be made according to the amounts IFN-gamma to be inactivated.
Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Please inquire for availability of azide free solutions
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
Specificity：Recognizes rat Interferon gamma (IFN-gamma) of both natural and recombinant origin. Inhibits the biological activity of natural and recombinant rat IFN-gamma. Specificity was further assessed by ELISA and Western Blot. No crossreactivities with other cytokines have been detected.
