84-1076-38　［取扱停止］Vimentin (FLJ36605, VIM) 500ul　V2122-11D

特徴

• Vimentin is an intermediate filament with a molecular weight of ~58kD, and an average diameter of 10nm.
• Within the same species, the central domain of the filament is 30% homologous to cytokeratin, desmin, neurofilaments, and glial fibrillary acidic protein.
• Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium.
• It is usually co-expressed with cytokeratin in some epithelial cells such as Sertoli cells.
• Vimentin expression may change as a tumor becomes more aggressive, as in the case of hyperplastic prostate where prostatic intraepithelial neoplasia strongly expresses vimentin (91%), but invasive carcinoma is minimally reactive with Mouse anti-Vimentin.
• Vimentin is useful as part of an antibody panel for differential diagnostic purposes.
• It may be included in an antibody panel for differentiating soft tissue tumors.
• For example, co-expression of vimentin and cytokeratin is indicative of epitheloid sarcoma.
• In one case, a differential antibody panel using vimentin, cytokeratin, CEA, and EMA distinguished reactive mesothelial cells from malignant epithelial cells in the cytodiagnosis of serous effusions in 39/46 cases (85%).
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1:200-400 for 30 min.
• No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues
• Optimal dilution determined by the researcher.
• Positive Control:Sarcoma and melanoma
• Cellular Localization:Cytoplasmic
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.