Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation.
The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome.
The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
Optimal dilution determined by the researcher.
Positive Control: Human brain from patient with Alzheimer’s disease
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human Ubiquitin.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full-length human ubiquitin. Cellular Localization: Nuclear, cytoplasmic and cell membrane
