Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta.
Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:200 for 30 minutes.
No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues
Optimal dilutions to be determined by the researcher
Positive Control:MAD109 cells, MCF-7 cells; Skin or lung
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Specificity：Recognizes human Tubulin beta. Blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Does not cause the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Species Crossreactivity: Bovine, Porcine, Rat, Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, Fungi, Sea urchin, Myxamoeba, and Plasmodium.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Ch Fr Gp Hu Mo Po Rt Ye
Immunogen：Synthetic peptide corresponding to aa416-430 of human tubulin beta. Cellular Localization: Cytoplasmic. MW: 55kD.
