Recognizes a protein of 145kD, which is identified as CD117/p145kit.
This rabbit polyclonal antibody does not interfere with the binding of SCF to c-kit.
It precipitates both the unoccupied as well as the occupied form of c-kit.
The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival.
C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the research
Positive Control: Stromal tumor, skin or tonsil.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Specificity：Recognizes human CD117. Species Crossreactivity: canine.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Ca Hu
Immunogen：Synthetic peptide corresponding to the cytoplasmic domain of human CD117/c-kit. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane. MW of Antigen: 145kD
