Osteopontin is a 34kD extracellular matrix protein with a cell-binding domain.
It was originally identified as a major component of the noncollagenous organic bone matrix however it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids.
It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation.
This antibody should prove useful in studying the expression of this matrix glycoprotein in tissue homeostasis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 2-4ug/ml for 2 hrs at RTImmunohistochemistry (Formalin/paraffin): 1:25.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells, Gastric carcinoma or Osteosarcoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human Osteopontin. Species Crossreactivity: porcine and canine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Po
Immunogen：Synthetic peptide corresponding to the internal domain of human Osteopontin. Cellular Localization: Cytoplasmic
