Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5.
Keratin 14 has been studied as a prognostic marker in breast cancer.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/ml for 2 hours at RT.
Detects a band at ~50kD.
Immunohistochemistry (Formalin/paraffin): 1:800-1:1600 for 20 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control:A431 cells; Skin or Squamous cell carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Specificity：Recognizes human Keratin 14.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from C-terminal of human Keratin 14. Cellular Localization: Cytoplasmic
