c-erbB-4 is the fourth member of class I receptor kinase family (ErbB).
HER-4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum.
Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDA-MB- 330 and MDA-MB-361.
Expression of erbB-4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3.
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Positive Control:T47D or MDA-MB-453 cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human c-erbB4. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1285-1308 from the C-terminus of human c-erbB-4/HER-4 oncoprotein. Cellular Localization: Cytoplasmic and cell membrane.
