MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells.
It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases.
Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (FFPE): 1:50-1:100 for 20 min at RT.
No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues
Optimal dilutions to be determined by the researcher.
Positive Control:MCF-7 cells, Breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.6, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes human Epithelial Membrane Antigen. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide from cytoplasmic tail of human Epithelial Membrane Antigen. Epitope: aa239-255. MW of Antigen: 265-400kD. Cellular Localization: Cytoplasmic and cell membrane.
