The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions.
Beta-catenin also binds to N-cadherin and co-immunoprecipitates with APC.
Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin.
Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml for 2 hours at RTImmunohistochemistry (Formalin/paraffin): 1:250 for 10 min at RT.
[Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]Optimal dilution determined by the researcher.
Positive Control: MCF-7 cells.
Breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Specificity：Recognizes beta Catenin. Species Crossreactivity: Human and Mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the C-terminus of human beta-catenin. Cellular Localization: Cytoplasmic and cell membrane
