The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors.
The expression of AR is reportedly inversely correlated with histologic grade i.e.
well differentiated prostate tumors show higher expression than the poorly differentiated tumors.
In prostate cancer, AR has been proposed as a marker of hormone-responsiveness.
Applications:Suitable for use in Immunofluorescence, ImmunoprecipitationWestern Blot and Immunoistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml for 2 hoursImmunohistochemistry (Formalin/paraffin): 1:100 to 1:200 for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be setermined by researcher.
Positive Control: LnCap cells or prostate carcinoma.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：rat
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 0.09% sodium azide
Specificity：Recognizes rat Androgen Receptor at ~110kD. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminal of rat AR. Cellular Localization: Nuclear
