84-1075-94　［取扱停止］Actin, Smooth Muscle 250ul　A0760-31D

特徴

• Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells, and also in tumors derived from these cells.
• Actins are highly conserved proteins expressed in all eucaryotic cells.
• Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement.
• Six distinct actin isotypes have been identified in mammalian cells.
• Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type.
• Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells.
• Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:25 for 30 minutes at RT No special pretreatment is required for staining of formalin/paraffin tissuesOptimal dilutions to be determined by the researcher.
• Positive Control:Smooth muscle of arteries, gut wall, uterus and leiomyosma
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.