XIAP is an endogenous inhibitor of cell death, which functions by suppressing caspases 3, 7, and 9.
The protein is also reported to play a a role in the activation of NF kappa B and the regulation of cyclins and cyclin-dependent kinase inhibitors (CDKIs).
Regulators of XIAP activity include XAF-1 and Smac.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/ml, detects a band of ~57kD in human kidney lysates
Immunohistochemistry (paraffin): 2ug/ml, Requires antigen retrieval using heat treatment prior to staining of paraffin sections
Optimal dilution determined by the researcher.
Recommended Secondary Reagents:Sheep anti rabbit IgG: HRP
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human X-chromosome linked inhibitor of apoptosis (XIAP). Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to a thirteen amino acid sequence with the C-terminal region of human XIAP.
