Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine.
The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain
Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000; Detects a band of ~60kD in rat caudate cell lysates
Immunohistochemistry (Frozen): 1:1000.
Suitable for use in paraffin sections
Immunofluorescence: 1:1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes Tyrosine hydroxylase (TH). Species Crossreactivity: rat and mammal
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Ma Rt
Immunogen：Tyrosine hydroxylase purified from pheochromocytoma.
