TNF alpha is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1-10ug/mlWestern Blot: 0.1-1ug/mlOptimal dilution determined by the researcher.
Endotoxin Level:<0.015EU/ug
Recommended Secondary Reagents:Sheep anti rabbit IgG: HRP
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes both native and recombinant equine TNF alpha. No detectable crossreactivity was observed with bovine, porcine, mouse or rat TNF alpha.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Eq
Immunogen：Recombinant protein corresponding to equine TNF alpha.
