TNF alpha is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.
Applications:Suitable for use in ELISA, Immunohistochemistry (frozen) and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot 0.1-1ug/mlOptimal dilution determined by the researcher.
Endotoxin Level:<0.015 EU/ug
Recommended Secondary Reagents:Streptavidin:HRP
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：rabbit
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
Specificity：Specific for equine tumor necrosis factor alpha (TNF alpha). Recognizes both native and recombinant equine TNF alpha. No detectable cross-reactivity was observed with bovine, porcine, mouse or rat TNF alpha.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Eq
Immunogen：Recombinant protein corresponding to equine TNF alpha.
