GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, detects a band of ~50kD in rat cortex lysates.
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM sodium fluoride should be added to the sample buffer.
Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background
Optimal dilution determined by the researcher.
Recommended Secondary Reagents:Sheep anti rabbit IgG:HRP
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat Growth Associated Peptide 43 (GAP-43), when phosphorylated at serine 41.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser41. Species sequence homology: human, bovine, chicken, canine, monkey, xenopus, zebrafish and mouse
