GluR1 is a component of AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid receptors), which play a key role at excitatory synapses.
GluR1 is widely expressed throughout the nervous system.
Phosphorylation of Ser831 and Ser845 is reported to potentiate GluR1 function, suggesting a possible role in synaptic plasticity, learning and memory.
Applications:Suitable for use in Western Blot and Immunohistochemistry (frozen).
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, detecs a band of approximately 100 kD in rat brain lysates
Optimal dilution determined by the researcher.
Recommended Secondary Reagents:Sheep anti rabbit IgG:HRP
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：150ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.
Specificity：Specific for glutamate receptor subunit 1 (GluR1) when phosphorylated at serine 831. The specifity of this antibody has been shown by blocking studies in Western Blotting, whereby staining was blocked by the phospopeptide. No blocking was seen by the corresponding non-phosphopeptide. Species Cross Reactivity: Human, mouse and rat (100% identity with immunogen sequence).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to amino acid sequence within GluR1 which includes phosphorylated Ser 831.
