Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis.
Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo.
Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry (frozen).
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, detects a band of ~95kD in rat hippocampus lysates.
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM sodium fluoride should be added to the sample buffer.
Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background
Optimal dilution determined by the researcher.
Recommended Secondary Reagents:Donkey anti sheep/goat IgG:HRP
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat Dynamin when phosphorylated at serine 774.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser 774. Species sequence homology: human, monkey, bovine, chicken, mouse and canine
