Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis.
Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo.
Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
Applications:Suitable for use in Western Blot and Immunohistochemistry (frozen).
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, detects a band of approximately 95kD in rat synaptosomal lysates
Optimal dilution determined by the researcher.
Recommended Secondary Reagents:Donkey anti sheep/goat IgG:HRP
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA, 50% glycerol.
Specificity：Specific for Dynamin when phosphorylated at serine 778. The specificity of this antibody has been shown by blocking studies in Western blotting, whereby staining was blocked by the phosphopeptide. No blocking was seen by the corresponding non-phosphopeptide. Species Cross Reactivity: Human, mouse & rat (100% identity immunogen sequence).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser 778.
