DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation.
As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine
The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (frozen): 1:1000Western Blot: 1:1000, detects a band of ~88kD in human caudate cell lysatesOptimal dilution determined by the researcher.
Recommended Secondary Reagents:Sheep anti rabbit IgG:HRP
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography from whole serum.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human dopamine transporter (DAT). Species Crossreactivity: Monkey
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：KLH-conjugated synthetic peptide corresponding to the C-terminus region (CT) of human dopamine transporter.
