DARPP-32 is also known as protein phosphatase 1 regulatory subunit 1B (PP1R1B).
DARPP-32 is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission.
DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation.
Phosphorylation at threonine 34 results in converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1).
Alternatively, phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Detects a band of ~32kD in rat caudate lysates
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat DARPP-32 when phosphorylated at threonine 34.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr 34. Species Sequence Homology: mouse, canine, human, bovine, chicken, and monkey.
