Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway.
Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w.
Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex.
Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000, detects a band of ~22kD in K562 and A549 whole cell lysates.
Immunohistochemistry (Paraffin): Requires antigen retrival using heat treatment prior to staining of paraffin sections.
Sodium citrate pH 6 is recommended for antigen retrival
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human BIM.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa22-40 of human BIM. Species Sequence Homology: mouse and rat.
