TdT is a nuclear enzyme that catalyzes the random addition of deoxynucleotide triphosphate to the 3’-OH groups of single-stranded DNA.
A 58kD immunoreactive peptide has been detected in human and mouse lymphoblastoid cell lines.
Also, a 58kD-60kD major peptide has been seen on Western Blots of mouse, rat, calf, feline and chicken thymus extracts.
Elevated activity of TdT has been shown in neoplasms derived from immature lymphoid cells, lymphoblastic lymphomas (LL), acute lymphoblastic leukemias (ALL), lymphoid blast crisis of chronic myeloid leukemias (CML), and lymphomas.
Rabbit anti-TdT may be used to distinguish LL from other lymphomas that do not express TdT.
Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded human tissue sections, or cell smears.
Epitope Retrieval Required, use Citrate buffer, pH 6.0
Enzyme Digestion is not required.
Optimal dilutions to be determined by the researcher.
Positive Control:Thymus
Expected Staining Pattern:Nuclear
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：bovine
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
